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ELISA原理與分類 [常見問題解答]

: 1.　ELISA的原理 ELISA的基礎是抗原或的固相化及抗原或的酶標記。結合在固相載體表面的抗原或仍保持其免疫學活性，酶標記的抗原或抗體既保留其免疫學活性，又保留酶的活性。在測定時，受檢標本（測定其中的抗體或抗原）與固相載體表面的抗原或抗體起反應。用洗滌的方法使固相載體上形成的抗原抗體復合物與液體中的其他物質分開。再加入酶標記的抗原或抗體，也通過反應而結合在固相載體上。此時固相上的酶量與標本中受檢物質的量呈一定的比例。加入酶反應的底物后，底物被酶催化成為有色產物，產物的量與標本中受檢物質的量直接相關，故可根[查看]; http://www.baichuan365.com/Article/356_1.html

ELISA中非特異性顯色原因分析 [常見問題解答]

: 【摘要】：影響ELISA中非特異性顯色的原因很多，如盒特異性、檢驗標本中含酶標記物的干擾物，操作過程中的問題。本文就這一原因作一簡要分析,以便可以通過以上措施把非特異顯色降至低限度，從而提高檢測的特異性，并得到更準確、可靠的實驗結果。【關鍵詞】 ELISA 非特異性顯色酶聯免疫吸附試驗（ELISA）自1971年自問世以來，以其敏感性高，特異性強，操作簡便、安全，而廣泛應用于臨床診斷，開創了免疫學診斷的新紀元。但同其它免疫診斷方法一樣酶免在它的研究、生產及使用中常常會碰到非特異性問題，本文就在實驗過程中[查看]; http://www.baichuan365.com/Article/355_1.html

ELISA實驗質量控制問題 [常見問題解答]

: 從1949年美國College of American Pathologists（簡稱CAP）首先開始研究臨床實驗室室內質量控制（簡稱質控）問題，美國學者Levery和Jenning于1950年發表了一篇關于使用質控圖的實驗室室內質控，臨床檢驗實驗室的室內質控工作正式拉序幕。到70年代，實驗室質量控制進入一個新的階段--質量管理，推行Good Laboratory Parctice（簡稱GLP）。進入80年代末期，GLP的統一標準產生了，發展到\"認證實驗室\"管理階段。[查看]; http://www.baichuan365.com/Article/353_1.html

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