D-果糖/D-葡萄糖檢測(cè)試劑盒 , D-Fructose plus D-glucose assay kit , 貨號(hào)：K-FRUGL
品牌：Megazyme
貨號(hào)：K-GLUGL
中文品名：D-果糖/D-葡萄糖檢測(cè)試劑盒
品名：D-Fructose plus D-glucose assay kit
規(guī)格：110 次檢測(cè) (手工) / 1100 (微孔板) / 1100 (自動(dòng)分析儀)
應(yīng)用：D-果糖/D-葡萄糖檢測(cè)試劑盒是一種酶法-紫外檢測(cè)方法，用于測(cè)量和檢測(cè)植物和食品中D-果糖,和/或D-葡萄糖含量。輔助因子更穩(wěn)定，溶解后在4°C下可保存超過1年。適合手工、微孔板和自動(dòng)分析儀分析。
原理：
檢測(cè)方法：分光光度法 @340 nm
反應(yīng)時(shí)間：~13min
檢測(cè)限：0.66 mg/L
應(yīng)用案例：
葡萄酒、啤酒、果汁、軟飲料、牛奶、果醬、蜂蜜、食療食品、面包、焙烘食品、糖果、甜點(diǎn)、糕點(diǎn)、冰淇淋、水果蔬菜、調(diào)味品、煙草、化妝品、藥品、造紙和其他原料(如生物培養(yǎng)、樣品等)
方法識(shí)別：
基于上述原理的檢測(cè)方法已經(jīng)被AOAC, EN, NEN, NF, DIN, GOST, OIV, IFU, AIJN, MEBAK和IOCCC等組織接受。
試劑盒組成：
| Bottle 1: | 緩沖溶液(15 mL, pH 7.5) 添加疊氮鈉(0.02%)作為防腐劑 |
| 4°C下穩(wěn)定超過 2年 | |
| Bottle 2: | NADP+, ATP和PVP。 |
| -20°C穩(wěn)定超過 5年 | |
| Bottle 3: | 己糖激酶和葡萄糖-6-磷酸脫氫酶懸浮液, 2.25mL. |
| 4°C下穩(wěn)定超過 2年 | |
| Bottle 4: | 磷酸葡萄糖異構(gòu)酶懸浮液(2.25 mL)。 |
| 4°C下穩(wěn)定超過 2年 | |
| Bottle 5: | D-葡萄糖和D-果糖標(biāo)準(zhǔn)溶液(5 mL, 每種糖濃度為0.2 mg/mL)。 |
| 4°C下穩(wěn)定超過 2年 |
優(yōu)點(diǎn)：
包含PVP可預(yù)防單寧抑制
經(jīng)過法國(guó)Suze la Rousse葡萄酒大學(xué)驗(yàn)證
價(jià)格非常具有競(jìng)爭(zhēng)力
所有試劑制備后可以穩(wěn)定保存超過2年（針對(duì)手工分析應(yīng)用）
在25°C或37°C下都能快速檢測(cè) 官網(wǎng)提供Mega-Calc™ 軟件工具用于一站式原始數(shù)據(jù)處理
包含標(biāo)準(zhǔn)溶液
輔助因子更穩(wěn)定
適合手工、微孔板和自動(dòng)分析儀分析。
經(jīng)過法國(guó)Suze la Rousse葡萄酒大學(xué)驗(yàn)證
價(jià)格非常具有競(jìng)爭(zhēng)力
所有試劑制備后可以穩(wěn)定保存超過2年（針對(duì)手工分析應(yīng)用）
在25°C或37°C下都能快速檢測(cè) 官網(wǎng)提供Mega-Calc™ 軟件工具用于一站式原始數(shù)據(jù)處理
包含標(biāo)準(zhǔn)溶液
輔助因子更穩(wěn)定
適合手工、微孔板和自動(dòng)分析儀分析。
參考文獻(xiàn)：
Grape and wine analysis: Oenologists to exploit advanced test kits. Charnock, S. C. & McCleary, B. V. (2005). Revue des Enology, 117, 1-5.
Megazyme “advanced” wine test kits general characteristics and validation. Charnock, S. J., McCleary, B. V., Daverede, C. & Gallant, P. (2006). Reveue des Oenologues, 120, 1-5.
Lactose fermentation by Kombucha – a process to obtain new milk–based beverages. Ili?i?, M., Kanuri?, K., Milanovi?, S., Lon?ar, E., Djuri?, M. & Malbaša, R. (2012). Romanian Biotechnological Letters, 17(1), 7013-7021.
Sourdough-leavened bread improves postprandial glucose and insulin plasma levels in subjects with impaired glucose tolerance. Maioli, M., Pes, G. M., Sanna, M., Cherchi, S., Dettori, M., Manca, E. & Farris, G. A. (2008). Acta Diabetologica, 45(2), 91-96.
Endocrine and metabolic effects of consuming fructose- and glucose-sweetened beverages with meals in obese men and women: Influence of insulin resistance on plasma triglyceride responses. Teff, K. L., Grudziak, J., Townsend, R. R., Dunn, T. N., Grant, R. W., Adams, S. H., Keim, N. L., Cummings, B. P., Stanhope, K. L. & Havel, P. J. (2009). Journal of Clinical Endocrinology & Metabolism, 94(5), 1562-1569.
Metabolic and endocrine profiles in response to systemic infusion of fructose and glucose in rhesus macaques. Adams, S. H., Stanhope, K. L., Grant, R. W., Cummings, B. P. & Havel, P. J. (2008). Endocrinology, 149(6), 3002-3008.
β-Fructofuranosidase and sucrose phosphorylase of rumen bacterium Pseudobutyrivibrio ruminis strain 3. Kasperowicz, A., Stan-Glasek, K., Guczynska, W., Pristas, P., Javorsky, P., Vandzurova, A. & Michalowski, T. (2012). World Journal of Microbiology and Biotechnology, 28(3), 1271-1279.
Comparative effects of fructose and glucose on lipogenic gene expression and intermediary metabolism in HepG2 liver cells. Hirahatake, K. M., Meissen, J. K., Fiehn, O. & Adams, S. H. (2011). PloS one, 6(11), e26583.
Measurement of glucose and fructose in clinical samples using gas chromatography/mass spectrometry. Wahjudi, P. N., Patterson, M. E., Lim, S., Yee, J. K., Mao, C. S. & Lee, W. N. P. (2010). Clinical Biochemistry, 43(1-2), 198-207.
Physiological studies of Leuconostoc mesenteroides strain NRRL B-1149 during cultivation on glucose and fructose media. Bivolarski, V., Vasileva, T., Shukla, R., Goyal, A. & Iliev, I. (2012). Journal of BioScience & Biotechnology, 1(3), 235-240.
Aldose reductase is implicated in high glucose‐induced oxidative stress in mouse embryonic neural stem cells. Fu, J., Tay, S. S. W., Ling, E. A. & Dheen, S. T. (2007). Journal of Neurochemistry, 103(4), 1654-1665.
Immobilised yeast grape must deacidification in a recycle fixed bed reactor. Portugal, I., Ribeiro, S. C., Xavier, A. M. R. B., Centeno, F. & Strehaiano, P. (2011). International Journal of Food Science & Technology, 46(2), 284-289.
The Elemental Composition of Synechocystis sp. PCC6803 under Nutrient Limiting Conditions. Blom, M. (2014). University of Amsterdam, Bachelor Thesis.
使用手冊(cè)：
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